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KMID : 0616619970030020707
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Journal of Soonchunhyang Medical College
1997 Volume.3 No. 2 p.707 ~ p.714
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The Studies for interaction of MMP-2 activating and nonactivating human breast cancer cell lines with Type Ⅰ collagen
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Abstract
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Thompson has previously shown that the vimentin-positive subset of human breast cancer cell lines, which are more invasive in vitro and in vivo, can activate MMP-2 when cultured on vitrogen gels, whereas the poorly invasive, poorly metastatic vimentin-negative subset cannot. In this study, we compared the interaction with type I collagen(vitrogen). The cell lines capable of responding to vitrogen for MMP-2 activations showed an enhanced ability to attach to vitrogen-coated culture wells. MCF-7(non-MMP-2 activating) and MDA -MB -231 (MMP -2 -activating) cells were selected for more detailed analysis. MDA-MB-231 cells showed a greater affinity for the 3-dimensional vitrogen than MCF-7 cells. Attachment of both lines to thin coatings of vitrogen was shown to require divalent cations, and to be mediated by 61 integrins. The a5 subunit, however, was shown to be involved in fibronectin attachment, but not vitrogen. The GRGDSP peptide dramatically inhibited fibronectin attachment of both cell lines, but also did not effect the vitrogen attachment of either. In contrast, the KGDEA recognition sequence for a2 integrin inhibited the attachment of MDA-MB-231 cells to vitrogen, but not fibronectin or laminin. These results show that the subset of human breast cancer cell lines which respond to the vitrogen gel with MMP-2 activation may interact more easily with the vitrogen, apparently through integrin-mediated recognition. Preliminary analysis reveals that the a2 ,61 integrin, previously implicated in vitrogen interactions may mediated this response.
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KEYWORD
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